A comparison of permeability of PSS/PAH, PVS/PAH, PSS/PDDA, and PVS/PDDA was made using 4000 MW FITC-dextran loaded for 36 h.Fig. Perfuse 12 mL of FITC-dextran solution at the rate of 12 mL per minute. . Our dextran is purified by size chromatography to remove unbound molecules and ensure proper cell biology analysis applications. Fluorophores. Synonym: FITC-Dextran MDL number MFCD00131092 Application Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals. Carefully remove the media from the insert so as not to touch or disturb the cell monolayer. Step 2. Gavage the mouse with 150 μl of 80 mg/ml FITC dextran (4kDa). FITC dextran is a green fluorescent dextran conjugate with excitation/emission wavelength around 495 nm/515 nm. FITC-dextran leakage was present in CLEC14A-KO mice after ischemia-reperfusion, and the numbers of tight junctional molecules were significantly decreased. Final molar F/P ratio was 0.06. Incubate the plate, protected from light, for 20 minutes at room temperature. Fluorescein isothiocyanate-dextran average mol wt 10,000 60842-46-8 Dissolve FITC-dextrans of different molecular weights (e.g., 4 kDa, 20 kDa, 70 kDa, etc.) Repeat for all mice. FITC-Dextran - Inhibitor - TargetMol Lencer WI, Weyer P, Verkman AS, Ausiello DA, Brown D.FITC-dextran as a probe for endosome function and localization in kidney. Surface bound virus fluorescence was quenched using a . FITC-Dextran is a marker consisting of coupling fluorescein-isothiocyanate to dextran. Molecular Weight. (Fig.1 1 D1,D2,E1,E2). Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of ... . Fig. Then, add mouse IgE to a concentration of one microgram per milliliter. Next, mix three milligrams of FITC-dextran powder with three milliliters of culture media. (Catalog #18770) and labeled with Anti-Dextran, Clone DX1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (open histogram). Trim the hair over the abdomen of a transplanted NSG mouse then clean the skin with All products from TargetMol are for Research Use Only. Sections were washed by covering them with saline for 30 s in order to dissolve the OTC compound before being coverslipped. PDF Fluorescein Isothiocyanate (FITC)- conjugated Antibodies FITC-dextran is supplied as a yellow/orange powder which dissolves freely in water or salt solutions giving a yellow solution. How can I label FITC to dextran? - ResearchGate

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